Microarray Gene Expression Data Society (MGED Society)
July 12, 2004

An open letter to the scientific journals:

A fundamental principle guiding the publication of scientific results is that the data supporting any scholarly work must be made fully available to the research community, in a form that allows the basic conclusions to be evaluated independently. In the context of molecular biology, this has typically meant that authors of a paper describing a newly sequenced genome, gene, or protein must deposit the primary data in a permanent, public data repository, such as the sequence databases maintained by the DDBJ, EBI, and NCBI. Similarly, we, members of the Microarray Gene Expression Data Society (MGED; http://www.mged.org), believe that all scholarly scientific journals should now require the submission of microarray data to public repositories as part of the process of publication. While some journals have already made this a condition of acceptance, we feel that submission requirements should be applied consistently and that journals recognize ArrayExpress (Brazma et. al. 2003), Gene Expression Omnibus (GEO) (Edgar et. al 2002), or CIBEX (Ikeo et. al. 2003) as acceptable public repositories. To this end, the members of MGED Society propose the following as a new paradigm for the publication of microarray based studies:

  1. Authors should continue to take primary responsibility for ensuring that all data collected and analyzed in their experiments adhere to the MIAME guidelines and continue to use the MIAME checklist (http://www.mged.org/Workgroups/MIAME/miame_checklist.html) as a means of achieving this goal.
  2. The scientific journals should require that all primary microarray data are submitted to one of the public repositories - ArrayExpress, GEO, or CIBEX - in a format that complies with the MIAME guidelines.
  3. The public databases should work with authors and the scientific journals to establish data submission and release protocols to assure compliance with MIAME.
  4. To assist with the review process, the databases should continue to work in collaboration with publishers to provide qualified referees with secure means of access to prepublication data. Authors should be strongly encouraged to submit data to the databases during review.

Naturally, data should be protected from general release prior to either publication or authorization from the data submitters whichever comes first. At a minimum, the journals should require valid accession numbers for microarray data as a requirement for publication and these accession numbers should be included in the text of the manuscript to allow members of the community to find and access the underlying data.

Since its inception in 1999, MGED has been working with the broader scientific community to establish standards for the exchange and annotation of microarray data. In December 2001, we proposed the 'Minimal Information About a Microarray Experiment' guidelines (MIAME; Brazma, Hingamp et al. 2001) and requested that interested parties provide feedback on its relevance and utility. The feedback from both researchers and scientific journals was overwhelmingly positive, yet almost everyone who responded also asked for help in implementing these guidelines.

Subsequently, in the summer of 2002, we submitted an open letter to various journals (e.g. Ball, Sherlock et al. 2002; Ball, Sherlock et al. 2002) urging the community to adopt the MIAME requirements for microarray data publication. We provided a checklist so that authors could ensure that sufficient information to allow their data to be re-analyzed by others would be available. Again, the response from the community was extremely positive and most of the major scientific journals now require publications describing microarray experiments to comply with the MIAME standards. While the adoption of these standards has greatly improved the accessibility of microarray data, much of it remains on individual authors' websites in a variety of formats; consequently, obtaining and comparing datasets remains a significant challenge. Clearly we need additional requirements for publication that include submission of expression data to public data repositories.

Though one might ask why this requirement was not part of the original MIAME recommendation, the answer is quite simple - MIAME was ahead of its time. While the major public DNA sequence database groups at the National Center for Biotechnology Information (NCBI) and the European Bioinformatics Institute (EBI) had developed nascent microarray data repositories, and work was underway to create a similar database at the DNA Data Bank of Japan (DDBJ), submitting data to these databases was a considerable burden for authors. However, since that time, improvements in the data-entry utilities available for the Gene Expression Omnibus (GEO; http://www.ncbi.nlm.nih.gov/geo), ArrayExpress (http://www.ebi.ac.uk/arrayexpress), and CIBEX (http://cibex.nig.ac.jp) databases, as well as a growing number of commercial and academic software packages capable of writing MAGE-ML documents (Spellman, Miller et al. 2002) that can be directly submitted to these public databases, have lowered the barriers for data submission to the point where we as a community must now reconsider that submission to one of these databases be a requirement.

Requiring authors to submit microarray data to the public databases will provide a number of distinct advantages to the entire research community:

In the same way that availability of sequence data had a profound impact on a wide range of disciplines, we believe that requiring that microarray data be deposited in public repositories as a necessity for publication will accelerate the rate of scientific discovery.

What this proposal requires is a change in the way in which we approach the publication of microarray-based studies. Both authors and journals have a responsibility to assure that the requisite data are available, and because submitting MIAME-compliant data can take considerable time and effort, this process should be factored into review and publication timelines. However, while this process may be time consuming and painful at first, we believe that the benefits of building an open repository of microarray data will far outweigh any initial disadvantages. As always, it is our sincere hope that these suggestions stimulate discussion within the community and that together we can arrive at a consensus that ensures that microarray data are widely and easily accessible. Finally we would like to urge the DDBJ, EBI and NCBI to work together towards exchanging all MIAME-compliant microarray data.

On behalf of MGED,

References:

PS. We would like to encourage the journals, referees and authors of microarray based papers to give us their feedback and to attend MGED 7 conference in Torronto September 8-10 (http://www.microarrays.ca/MGED7.html) where the described policies will be discussed in a session devoted to MIAME.